Mitegen plastic capillaries can actually last about a week from a
Mitegen seminar I attended. I also got ice when I tried to freeze it
once and I didn't further pursue it. Maybe coat the capillary with
glycerol will help?
Nian Huang, Ph.D.
UT Southwestern Medical Center
On Thu, Feb 17, 2011 at 11:03 AM, R Conners, Biochemistry
<[log in to unmask]> wrote:
> Dear all,
>
> We are working on a Category 3 protein which must be contained so we have
> our crystals mounted in a loop and then covered with a plastic Mitegen cover
> which is glued in place. We're currently collecting at room temperature, but
> wondered if anyone has any experience of using a contained system at low
> temperatures? Any attempts I've had so far at freezing through either the
> plastic or a glass capillary have resulted in formation of ice on the
> surface so it is not even possible to see the crystal to centre it.
>
> Best wishes,
>
> Becky
>
> -------------------------------------------------
> Dr Becky Conners
> School of Biochemistry
> University of Bristol, UK
>
> http://www.bris.ac.uk/biochemistry/brady
> [log in to unmask]
> 0117 3312149
>
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