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Subject:
Re: off-topic: tag removal
From:
Peter Hsu <[log in to unmask]>
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Date:
Thu, 24 Feb 2011 18:15:52 +0000
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I haven't used it personally, but I've heard some people engineering a his SUMO tag on to the protein and then use SUMO protease to cleave it off. The protease apparently makes very clean cuts and no extra residues are left. Best of luck, Peter
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