> 2) Scale F-holo and F-apo. Use "Experimental Phasing", "Data
> Preparation", "Scale and Analyse Data Sets", "Scale refinement using
> Scaleit". Don't include anomalous differences unless your interest is in
> changing anomalous scatterers. My notes indicate that Fhscal works
> better but does not have anisotropic scaling. If your two data sets do
> not differ anisotropically try Fhscal.
This is not a problem. First scale anisotropically with your
favourite program. Then the Kraut scaling correction using FHSCAL is
a small correction on top of this, so just rescale the
anisotropically-scaled output using FHSCAL. I didn't include an
anisotropic scaling option in FHSCAL for the simple reason that this
option was already available in other programs.
> The greater the difference in cell constants the greater the "noise" in the
> map. I think the high resolution cutoff for the maps should be 2 A
> delta/(A+delta) where A is the cell edge with the largest change, and delta
> is the amount of change (in Angstrom).
> Basically a 1A change for a 100A edge would require a 2A resolution limit.
> A 5A change would imply a 10A cutoff and a very boring map. I would
> appreciate feedback on this procedure, if you find it hard to understand or
> it doesn't work. Certainly the Phenix solution looks simpler. Dale
See this thread:
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-- Ian
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