Hi Gloria,
Accelagen offers TurboTEV (http://www.accelagen.com/TurboTEV-detail.htm) at
an affordable price. Please contact us for bulk order discount.
TurboTEV has dual GST- and His-tags and has high specific activity. In our
experience, a 1:50 (w:w) ratio cleaves most of the tags. 1:100 ratio works
in most cases. We have a protocol on the product website as well.
In addition, we offer TurboNuclease
(http://www.accelagen.com/TurboNuclease-detail.htm), a benzonase equivalent
nuclease, for reducing lysate viscosity.
Chun
Accelagen
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Gloria
Borgstahl
Sent: Friday, January 14, 2011 6:36 AM
To: [log in to unmask]
Subject: [ccp4bb] TEV protease
We are using TEV protease to make a big batch of protein for
structural studies. We usually use thrombin, so this is our first
time using this enzyme on a large scale. Boy is it expensive! Does
anyone know of a bulk source for this enzyme and what ratios of use do
you recommend. Imidazole inhibits?
Many thanks. Gloria
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