Hello Qing Chen,
if by 'shift' you mean the actual coordinates you can do in coot as Herman
describes. If you want to shift the sequence numbering: Coot also has an option
to renumber a sequence by an offset and the offset can also be negative.
Cheers, Tim
On Thu, Jan 13, 2011 at 12:27:49PM +0100, Qing Chen wrote:
> Let's say I need to insert two G between resi 98 and 99. I tried in the way
> as Herman suggest: break the chain in the domain boundary, add terminal
> residues at the N-terminus of the 2nd domain. But then there is no space to
> accommodate the inserted residues, the two glycerin backbones overlap with
> resi 97 and 98. Is there a way to shift the whole 1st domain a bit ?
>
>
>
> On Thu, Jan 13, 2011 at 12:16 PM, <[log in to unmask]>wrote:
>
> > use coot: calculate - model/fit/refine - add terminal residue.
> > this will add alanines. you can change these to glycines with the simple
> > mutate option in the same pop up menu.
> >
> > best,
> > HErman
> >
> > ------------------------------
> > *From:* CCP4 bulletin board [mailto:[log in to unmask]] *On Behalf Of *Qing
> > Chen
> > *Sent:* Thursday, January 13, 2011 11:58 AM
> > *To:* [log in to unmask]
> > *Subject:* [ccp4bb] creat a model with insertion
> >
> > Dear all,
> >
> > I have the wt protein structure that contains two domains. I want to creat
> > a model with two glycerin inserted between the two domains.
> > Which software or webserver could do this?
> > Pls help.
> >
> >
> > Qing Chen
> >
> >
--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
phone: +49 (0)551 39 22149
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