As Sacha suggests, if you were working on the ribosome, then MR at 8.5A would probably be straightforward. But what is important is the ratio of the size of the molecule to the resolution limit, and for molecules of a typical size this resolution is almost certainly too low. It basically comes down to questions of how much information you have, which you can think about in terms of how distinctive the shape is at your resolution or even how many reflections you have.
Best wishes,
Randy Read
On 20 Jan 2011, at 13:35, Simon Kolstoe wrote:
> Dear CCP4bb,
>
> I've just started on a new project and was rather excited to see protein spots for my first few crystals at Diamond the other day. The only problem is that the reflections only went out to 8.5A. As it should be possible to get a solution using molecular replacement (and whilst I am waiting for new and hopefully better crystals to grow) will MR work with only 8.5A data (100% complete)? I've spent the last couple of days playing with phaser and molrep but not had much luck so far, so was wondering if 8.5A might be a lost cause for MR?
>
> Thanks,
>
> Simon
------
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: + 44 1223 336500
Wellcome Trust/MRC Building Fax: + 44 1223 336827
Hills Road E-mail: [log in to unmask]
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
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