Dear All,
Please see the maps for the ASN residues of the protein, As suggested by
colleague, they are NAG which are connected to the N atom of ASN413 & ASN611.
I put the NAG molecule to those density and try to use the following command
line in PDB,
LINKR C1 NAG A1003 ND2 ASN A 611 1555 1555 1.45
but after restrained refinement using REFMAC5 (quite latest version), the bond
distance between N of ASN and C1 of NAG seems to be off (1.28 A) from the value
I set for this restraint.
My question is related to the true command of bond restraint for this
situation?
Also if I want to show a "link or line" between N atom of the ASN and C1 atom of
NAG in COOT, any idea how to do this?
many thanks in advance
stephen
--
Dr. Stephen Sin-Yin Chui
Research Assistant Professor,
Department of Chemistry,
The University of Hong Kong, Pokfulam Road,
Hong Kong SAR, China.
Tel: 22415814 (Office), 22415818 (X-ray Diffraction Laboratory)
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