I don't know how many of these crystals you have, but if you can spare one try freezing it straight out of the drop without cryoprotection. Certain salts can act as cryoprotectants at high enough concentrations. I don't know about phosphate salts, but I've had crystals that grew in 2.5M ammonium sulfate which I froze without cryoprotection and rarely saw an ice ring in my diffraction.
Good luck,
Mike
----- Original Message -----
From: "Jerry McCully" <[log in to unmask]>
To: [log in to unmask]
Sent: Wednesday, December 15, 2010 1:13:09 PM GMT -08:00 US/Canada Pacific
Subject: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium hydrogen phosphate
Dear All;
Recently we got some crystals from the condition #51 in the SaltRx crystallization kit from Hampton research.
It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to do a test diffraction ASAP.
What cryoprotectant did you use for this condition?
Thanks a lot and have a nice holiday season!
Jinghua
--
Michael C. Thompson
Graduate Student
Biochemistry & Molecular Biology Division
Department of Chemistry & Biochemistry
University of California, Los Angeles
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