Hello:
I am making a kinase in e.coli that seems to autophosphorylate itself a bit, which makes it difficult to get a pure homogeneous sample for crystallization. I would like to use a phosphatase to remove the abberant phosphorylation. I have seen lambda phosphatase used for this type of thing in the past, but buying it is pricey. Does anyone have a e coli construct for this or some other phosphatase or perhaps another suggested solution to this dilema? thanks in advance.
-Tom
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