On Fri, 2010-12-10 at 19:32 +0100, Petr Kolenko wrote:
> But now I have very low RMSDs. And then you read in this
> forum quite often, that we should refine against the data and not
> against the restraints. :)
Well, at 3.6A you must have low rmsds. And at 3.6A you don't really
have any data to refine against beyond backbone conformation and maybe
some sidechain orientation. You have perhaps 4-5 reflections per
residue, right? So you barely have enough to identify backbone torsions
with any degree of confidence. We should, of course, refine against the
data, but not against the noise in it.
Cheers,
Ed.
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
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