Dear Simon,
Not sure I understand this. You have three different peptides in your
sample? Do you also have three different chains in your MolSystem (you
should)? Are you assigning the different peaks to different chains? Do all
the peptides have a Pro-4? Why are you trying to assign a new resonance to
something that is already assigned elsewhere, instead of assigning the
peak to the same pre-existing resonance?
Some more details might help,
Rasmus
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Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Tue, 23 Nov 2010, S.P. Skinner wrote:
> Hi All
>
> I am using a 1H-13C HSQC spectrum of three separate molecules all in one. I
> am attempting to assign more than one peak to a C-H resonance. When I
> attempt to assign a resonance in a sister list of a spectrum, that has
> already been assigned previously in another list, the message: Redundant
> resonance: There are more resonances (3) than atoms set for 4Pro HD3/HD2.
> The reason for multiple assignment is that the three different peptides have
> different intensities and therefore require their own assignment.
>
> Any suggestions on how to circumvent this problem?
>
> Kind Regards
>
> Simon Skinner
>
> --
> Simon P Skinner
> Protein Chemistry Group
> Leiden Institute of Chemistry, Universiteit Leiden
> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
> E-mail : [log in to unmask]
>
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