Dear Lisa,
I do not think that any current prototype fits the bill. I shall have to
add one, it sounds like it would be a bit complex. Could you send me
either a litterature reference (prferable), and/or a more detailed
description of the magnetisation transfer path. Is Cmethyl
sometimes/Always/never the same as Cg? DOes the magnetisation start and
end on Hmethyl? Is this a relayed hard-pulse transfer, or a TOCSY
transfer? etc.
Yours,
Rasmus
---------------------------------------------------------------------------
Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Tue, 2 Nov 2010, Lisa Warner wrote:
> Hi Rasmus,
>
> On a related note, I am wondering which prototype I should be using for an HMCMCGCBCA Where the
> acquired dimensions are F1=cgamma, cbeta, calpha, F2=cmethyl, F3=hmethyl.
> thanks -- lisa
>
> On Mon, Nov 1, 2010 at 11:45 AM, Rasmus Fogh <[log in to unmask]> wrote:
> Dear Justin,
>
> >From what you say, ExpPrototype no. 15, 'H_[N].Jmultibond' ought to do. That gives a HN
> 2D hsqc with transfer through multibond couplings only. Does taht sound right, or is
> there more to your experiment than that?
>
> Yours,
>
> Rasmus
>
> ---------------------------------------------------------------------------
> Dr. Rasmus H. Fogh Email: [log in to unmask]
> Dept. of Biochemistry, University of Cambridge,
> 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
>
>
> On Thu, 21 Oct 2010, Justin Lecher wrote:
>
> Hi all,
>
> I am looking for a experiment prototype of a long range HSQC where the
> side chain H-C protons are correlated with the sidechain N in Histidins.
> Is this already included?
>
>
> justin
>
> --
> Justin Lecher
> Institute for Neuroscience and Biophysics
> ISB 3 - Institute for Structural Biochemistry
> Research Centre Juelich
> 52425 Juelich, Germany
> phone: +49 2461 61 2117
>
>
>
>
>
>
> --
> Lisa R. Warner
> Graduate Student
> Department of Chemistry and Biochemistry
> 215 UCB
> University of Colorado, Boulder
> Boulder, CO 80309-0215
> [log in to unmask]
> 303.492.8085
>
>
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