Is it known/can you divulge the nature/structure of the macromolecules in
the crystal? Is it protein, nucleic acid, other? Does the structure have
periodicity to it?
JPK
----- Original Message -----
From: "David Goldstone" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, November 03, 2010 10:53 AM
Subject: Re: [ccp4bb] Strange spots
> Dear All,
>
> Thank you for the replies sorry about the delay in my reply. Here is some
> more information, for those of you that are interested, to try fill in
> some gaps.
>
> The data was collected on our home source with osmic vairmax-HF optics and
> an RAXISIV++ detector. We are investigating whether it is an optics issue
> but this is unlikely as other crystals in the screening run didn't display
> this phenomenon.
>
> The crystal was grown in the presence of 12% glycerol and transfered to
> 20% glycerol as a cryo. I haven't tried other cryos as crystals also grow
> in 20% glycerol and do not require further cryoprotectant.
>
> I have uploaded a movie showing a wedge of data showing how the circles
> around the spots progress.
> http://www.4shared.com/video/o8_YmInD/Spot_defect.html (~12mb download)
>
> The crystals index and scale in p6122 (a=b=73, c=110) with a single
> monomer in the ASU (by matthews, 45% solvent). We do however see a peak in
> the native patterson at (0,0,0.2 ~50% origin height).
>
> Cheers
>
> Dave
>
>
> On 29/10/2010 17:08, David Goldstone wrote:
>> Dear All,
>>
>> Does anyone have any insight into what the circles around the spots
>> might be?
>>
>> cheers
>>
>> Dave
>
> --
> David Goldstone, PhD
> National Institute for Medical Research
> Molecular Structure
> The Ridgeway
> Mill Hill
> London NW7 1AA
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Northwestern University
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