Hello Eric,
I would try not to store a protein for 3-4 days and set up drops as quickly as
possible. 3-4 days can be a long time for a sensitive protein. If you expressed
too much, add 15% glycerol and store the protein at -80deg. Gel-filtrate the
solution and re-concentrate before the next use.
Tim
On Fri, Nov 05, 2010 at 08:40:51PM +0000, <Eric Karg>
wrote:
> Dear all,
>
> I'm working on a protein which starts to precipitate after 3-4 days of storage at 4 degrees or room temperature. The storage buffer contains 300 mM NaCl because at lower salt concentrations it also tends to precipitate. Different buffers and adding glycerol did not help although this was not done in a systematic way. Has anyone had similar experiences? Any suggestions to overcome this problem?
>
> Thanks in advance!
>
> Eric
--
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Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
phone: +49 (0)551 39 22149
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