Dear Daniel,
probably the first and easiest thing you could check is whether the freezing
conditions are suboptimal, by a room temperature measurement.
If that fails, you can add another purification step to your complex (certainly
worth a try), and/ or a restricted proteolysis, which might chop off some floppy
loops without disrupting the complex.
You might also try to solve the structure at 8A - seems possible if I remember
correctly from the ECM...
Tim
On Thu, Sep 09, 2010 at 04:23:30AM +0900, Daniel Lee wrote:
> Hi Cyrstallographers....
>
> I made the crystal of triple complex and the M.W is about 500 kDa.
> However, the diffraction is about 8 A.
> At first, the crystal form was needle. the needle form was changed by
> addtive screening.
>
> the crystal condition is under 1 M ammonium formate with Hepes (pH 7.5).
> Crystal size is more than 0.4 um.
>
> I tried to do annealing, dehydration and glutaraldehyde use but It
> does not increase the diffraction.
>
> I need some help.
>
> I will very appreciate your all comments.
>
> Thanks
>
> Best,
> Daniel
--
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Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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