Hi Cyrstallographers....
I made the crystal of triple complex and the M.W is about 500 kDa.
However, the diffraction is about 8 A.
At first, the crystal form was needle. the needle form was changed by
addtive screening.
the crystal condition is under 1 M ammonium formate with Hepes (pH 7.5).
Crystal size is more than 0.4 um.
I tried to do annealing, dehydration and glutaraldehyde use but It
does not increase the diffraction.
I need some help.
I will very appreciate your all comments.
Thanks
Best,
Daniel
|