POST-DOCTORAL POSITION
DATE OF AVAILABILITY : starting as soon as possible, for 2 years (ANR
contract).
TITLE : purification and crystallization of ABC transporters
POSITION: the position is opened at the Institute for Structural Biology
(IBS) in Grenoble in the team of Jean-Michel Jault and will be carried
out in the framework of a collaborative project between chemists and
biologists, both in Lyon (IBCP and UCB) and in Grenoble (IBS and UJF)
with funding from an ANR grant.
JOB DESCRIPTION: Multi-Drug Resistance (MDR) is an increasingly
challenging problem in the biomedical field and is widespread from
tumour cells to microbes. In bacteria, resistance to multiple
antibiotics is often associated with the presence of MDR pumps and
several of these belong to the largest superfamily of membrane proteins,
the ABC (“ATP-Binding Cassette”) transporters, which include some human
members whose dysfunction is involved in severe pathologies (cancer,
cystic fibrosis, adrenoleukodystrophy…). This project will focus on two
bacterial ABC transporters that we routinely purify in high yields in
their functional states, with the goal to crystallize them and solve
their 3-D structure, using both classical detergents and newly
synthesized ones.
JOB REQUIREMENTS: the applicant should hold a PhD in Biochemistry and
have a strong background in protein biochemistry (purification and
biochemical/biophysical characterisation) and in protein
crystallography. Additional knowledge in "membrane proteins" will be
appreciated.
SCIENTIFIC ENVIRONMENT: the Institut de Biologie Structurale (Institute
for Structural Biology) is located in the Grenoble “Polygone
Scientifique” area, in the close vicinity of the EMBL outstation, ESRF
and ILL, i.e. it is one of the most renowned scientific area in France
for biological research. At the IBS, the post-doc will benefit from a
technical environment devoted to purification, characterization and
crystallization of membrane proteins (MP3, PAOL and high throughput
membrane protein crystallization platforms), as well as the scientific
expertise associated with these. More information available from
http://www.psb-grenoble.eu/ and http://www.ibs.fr/?lang=en
TO APPLY: please send a CV and the names of two referees to Jean-Michel
Jault : [log in to unmask]
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