Hi all,
I am using CCPN to auto-assign a protein for which I have manually
picked peaks. I have HNCA, HNCOCA, HNCACB, CBCACONH, and 15N HSQC
spectra. I have curated a nice list of peaks and followed the
included instructions closely.
However, when I run Nexus, I get the "Preparing Spin System Typing"
progress bar stuck at zero. Then, I get the following error:
Exception in Tkinter callback
Traceback (most recent call last):
File "/Applications/ccpnmr2/python2.6/lib/python2.6/lib-tk/Tkinter.py",
line 1410, in __call__
return self.func(*args)
File "/Applications/ccpnmr2/ccpnmr2.1/python/ccpnmr/nexus/AutoBackbonePopup.py",
line 1297, in runNexus
keepExisting, progressBar)
File "/Applications/ccpnmr2/ccpnmr2.1/python/ccpnmr/nexus/AutoBackboneNexus.py",
line 132, in autoBackboneNexus
progressBar)
File "/Applications/ccpnmr2/ccpnmr2.1/python/ccpnmr/nexus/AutoBackboneNexus.py",
line 497, in getInitialAssignMatrix
scores = getShiftsChainProbabilities(shifts, chain)
File "/Applications/ccpnmr2/ccpnmr2.1/python/ccpnmr/analysis/core/ChemicalShiftBasic.py",
line 232, in getShiftsChainProbabilities
total = sum(probDict.values())
TypeError: unsupported operand type(s) for +: 'float' and 'NoneType'
Nexus works with another, old dataset, so I suspect it is specific to
the current project. Any ideas what could be the matter? I do have a
phosphoserine residue in the sequence, could this be the issue?
Thank you in advance for your help!
~Jeremy
|