If you are using malachite green assay, there will be an issue with the solution turning green at high ATP concentrations. There is a protocol to reduce ATP hydrolysis after the addition of malachite green (http://www.ncbi.nlm.nih.gov/pubmed/16674910). An alternative to malachite green assay is MESG assay.
Good luck!
>>> dengzq1987 <[log in to unmask]> 08/29/10 3:00 AM >>>
Dear all,
recently, i prepared the ATP solution used to assay ATPase activity. when the Control Well just containing assay buffer and ATP mixed with molybdate ,the colour changed to green, this mean that there is free pi in the
solution.the assay buffer and water is free from pi.and the ATP stock solution has free pi ,maybe the ATP hydrolysis when prepared .any suggestion about preparing ATP stock solution is appreciative.
Best regards!
2010-08-29
dengzq1987
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