Hello,
If the data are being collected at cryogenic temperatures and no inhibitor has been included in the cryoprotectant, perhaps the inhibitor would diffuse out of the active site?
Ping
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T z u - P i n g K o < [log in to unmask] >
Institute of Biological Chemistry
Academia Sinica, Nankang, Taipei 11529, Taiwan
Tel. +8862 2785 5696 ext 5011 FAX. +8862 2788 9759
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>Date: Sat, 28 Aug 2010 13:40:11 +0800
>From: CCP4 bulletin board <[log in to unmask]> (on behalf of J¤p. <[log in to unmask]>)
>Subject: [ccp4bb] Problem with getting protein-inhibitor complex structure
>To: [log in to unmask]
>
>Dear all,
> We are working on protein-inhibitor complex crystal structures, although we did get nice crystals and soaked them with inhibitor for several days, all structures we obtained were empty!!There are nothing at the active site. For several crystals. their color turned very clearly from colorless to yellow the color of the inhibitors, nothing found either.
> These inhibitors work very well in enzyme assay. We, especially our students, are very depressed...
> Any suggestions will be really appreciated!
>
>
>
>
>Xiaopeng Hu, D.Sc.,
>School of Pharmaceutical Sciences
>Sun Yat-sen University,
>Higher Education Mega Center
>Guangzhou, Guangdong, China 510006
>Tel. +86 020 39943032
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