Hi Qing,
just want to add that it also may depend on the redundancy of the data.
While good MAD experiments were performed with low redundancy (best to
use a strategy for data collection) a successful SAD experiment often
depends on high redundancy.
Best Regards,
Georg
Am 30.08.2010 20:58, schrieb Phoebe Rice:
> Dear Qing,
> Many structures have been solved that way. Make sure you try solvent modification (flattening / flipping) on your map. This is because SAD will give two equally-probably estimates for the phase. The initial, unmodified map will use the average of the two, but solve modification should be able to break the amibiguity.
> It sounds like you're new to crystallography - do you have a local advisor to get help from?
> Phoebe
>
> =====================================
> Phoebe A. Rice
> Dept. of Biochemistry& Molecular Biology
> The University of Chicago
> phone 773 834 1723
> http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
> http://www.rsc.org/shop/books/2008/9780854042722.asp
>
>
> ---- Original message ----
>
>> Date: Mon, 30 Aug 2010 20:32:26 +0200
>> From: CCP4 bulletin board<[log in to unmask]> (on behalf of Jane Bailey<[log in to unmask]>)
>> Subject: [ccp4bb] About SAD phasing
>> To: [log in to unmask]
>>
>> Hi, All
>>
>> I would like to ask whether is it possible to
>> allocate the Se site and obtain the phase by just
>> using the SAD data set (no native dataset used)?
>>
>> Thanks,
>> Qing
>>
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