Hello Qiangmin,
Here are links to a few web resources that have tips for membrane protein crystallization that may help with your optimization strategy:
http://kb.psi-structuralgenomics.org/update/2009/06/full/th_psisgkb.2009.25.html
http://web.emeraldbiosystems.com/blog/bid/33916/8-Practical-Tips-for-Membrane-Protein-Crystallization
http://mcl1.ncifcrf.gov/nihxray/Tips-and-Tricks_Crystallization_Membrane_Protein.pdf
good luck,
Eric
__________________________
Eric Larson, PhD
Biomolecular Structure Center
Department of Biochemistry
Box 357742
University of Washington
Seattle, WA 98195
On Mon, 30 Aug 2010, qiangm zhang wrote:
> Hi all,
> I got a crystal of one membrane protein (~60kD) from Na/K phosphate condition (see getit_4), then I got the improved crystal
> like getit_5 after trying seeding, different detergents, lipids and additives. But this crystal does not diffract at all, I already
> tried Izit staining which shows it is protein crystal (detergent crystal?). Does anyone have any good suggestions for the
> optimization of this membrane protein crystallization? Thank you in advance.
>
> Best regards
>
> Qiangmin Zhang
>
> Biomedical Science Tower 3
> Room1034
> 3501 Fifth Avenue
> Pittsburgh, PA 15260
>
>
>
>
>
> --
> 张强敏
>
>
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