I think you need a special trick to express C-type heme proteins.
For B hemes (protoheme, protoporphryn 9), which I think is
what the P450's have, E.coli is always expressing several
proteins which contain it. But I guess there may be some way
to up the production in case of an overexpresser.
Hari Namboodiri wrote:
> Hi CCPers
>
> Can anyone provide insights about expressing heme containing proteins in E.col? Does E.coli need any porphyrin precursor during expression or you need special E.coli strains. I have references mentioning delta-aminolevulinic acid for one ortholog but none for another. The enzyme is CYP51.
>
>
> Thanks
> Hari
>
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