Hello Kornelius,
In 2 or 3 cases I successfully used a homology model for molecular
replacement using a model generated from the PHYRE server
http://www.sbg.bio.ic.ac.uk/~phyre/.
I know it is not the same as ROSETTA but if there is some sequence homology
you might be lucky...
Best regards
Eike
Am 25.08.10 10:13 schrieb "Kornelius Zeth" unter
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> Dear all,
>
> I was wondering if anybody has used the ROSETTA software to generate a MR
> model that could subsequently being used successfully for a MR solution case.
>
> The sequence of the protein we work with is relatively small, ~ 85 residues.
> Crystallization is not very reproducible. Resolution is 1.9 A. Crystals are
> extremely rare.
>
> I would be grateful for any hints and will send a summary of all personally
> sent comments to the list.
>
> Thank you and have a nice day
>
> Kornelius
>
> ----------------------------------------------
> Kornelius Zeth
> Max Planck Institute for Developmental Biology
> Dept. Protein Evolution
> Spemannstr. 35
> 72076 Tuebingen, Germany
> [log in to unmask]
> Tel -49 7071 601 323
> Fax -49 7071 601 349
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