Check the thermal stability with and without your ligand. You could do this via CD or with the help of Sypro Orange in a RT-PCR machine.
Jürgen
......................
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.mac.com/bosch_lab/
On Aug 4, 2010, at 4:10, Xuan Yang <[log in to unmask]> wrote:
> Dear All,
>
> 3D structure modeling server I-TASSER predicts a binding site for NADPH and I want to test this prediction. What would be the nice quick way to tell whether this protein bind NADPH or not, when I have a lot of recombinant protein?
>
> Sincerely,
>
> Xuan Yang
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