Vandana,
Roger gives some good advice and I agree with Fred about not
knowing the number of mols in the AU. I routinely see solvent content in
the 75% range for many different crystals, although they tend to
diffract rather poorly. In addition, you may have to convert your search
model to poly-alanine and you may want to decrease the resolution during
MR.
Jon
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
Argonne, IL 60439
email: [log in to unmask]
Tel: (630) 252-0682
Fax: (630) 252-0687
> Vandana Kukshal wrote:
>> hello sir ,
>>
>> recently i have collected one data of 3.0 A of a protein having no
>> sequence homology with any known PDB .
>> but
>> while fold prediction i got 100 % identical fold with some of the
>> protein .
>> space group of my protein is P622 and showing 6 molecule in a
>> assymetric unit.
>> the homologous fold proteins are trimer protein.
>> can i run MR in this case . how i should proceed.
>> i m trying for MIR also .
>> regards
>> vandana
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