Hi James,
I had similar problems with MRC maps. As a solution, a colleague of
mine, working with EM, read the MRC map into SPIDER and saved it as a
CCP4 map. That worked for me. Unfortunately, my colleague is absent for
some time, and I'm still not familiar with EM software. But maybe, you
find a colleague at your institute to help you with that.
Good luck,
Dirk.
Am 13.07.10 14:32, schrieb James Whittle:
> Hi all-
>
> I'm trying to convert a cryoEM map from FREALIGN for use with various
> CCP4 programs, or with MAPMAN. Even though the MRC format is derived
> from the CCP4 map file format, every program I've tried fails to open
> it, (except for Chimera which displays the map but cannot save it to
> another format for lack of memory).
>
> For example, in MAPMASK I get the error:
>
> >>>>>> CCP4 library signal ccp4_map:No associated header (Error)
> raised in ccp4_cmap_open <<<<<<
> >>>>>> CCP4 library signal ccp4_map:Cannot open file (Error)
> raised in MRDHDS <<<<<<
>
> or with MAPMAN:
>
> **CCPOPN ERROR** FORMATTED OLD file open failure on unit 1
> Logical name: DLP_100.map, File name: DLP_100.map
> Cannot send after transport endpoint shutdown
>
> ERROR --- While reading map header. Sorry !
> ERROR --- While opening map file
>
>
>
> Is there a way to re-write the header to be compatible with CCP4?
>
> --James
--
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Dirk Kostrewa
Gene Center Munich, A5.07
Department of Biochemistry
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Germany
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