Petr Kolenko wrote:
> Dear all,
>
> I am working on crystallization of a protein purified using
> intein-chitin binding domain. There is 86% identity with protein with
> already known high-resolution structure, but I am not able to get any
> crystals. I heard that reversed phase chromatography is not good for
> crystallization, e.g. Has anyone of you observed something similar for
> intein-chitin chromatography? Is there any serious "structural" damage
> to the target protein during the purification?
>
> --
>
>
> Petr Kolenko
> [log in to unmask] <mailto:[log in to unmask]>
> http://kolda.webz.cz
The failure in crystallisation can be due to so many reasons...
including protein damage (ending up with a mixture of components is
probably one of the worse cases) Sometimes you need to modify your
protein in order to crystallise it, or even use an analog from another
organism.
Fred.
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