I would normally do the second, unless they are pretty clearly nonisomorphous. If you combine them in Pointless, this will reset the batch numbers automatically.
Phil
On 2 Jul 2010, at 05:35, Fengyun Ni wrote:
> Hi all,
>
> I have a question on how to combine two dataset from different crystal of
> the same
> protein.
>
> The first way I could think of is that,
> 1) Merge two dataset seperately;
> 2) Scale them to see whether they are consistent with each other enough as
> indicated by
> the R-factor;
> 3) If the R is low enough, say below 10 or 15%, then take the average for
> both the F and
> SIGF.
>
> The other way is that,
> 1) Take the unmerged file from MOSFLM, and reset their batch number;
> 2) Run SCALA to scale these two unmerged dataset at the same time.
>
> Could anyone tell which way is the better or the correct way?
> Thank you in advance!
>
> --
> alphar
|