The rnase structure used as the $CEXAM for CCP4 is another example - a
typical coordinate set is 2sar.pdb
There one monomer binds the substrate very clearly, whilst the other is
blocked by crystal contacts.
Eleanor
ANDY DODDS wrote:
> Hello,
>
> I am solving a structure of an enzyme, which crystallises as a dimer.
> We have pretty good evidence that this operates as a dimer in vitro,
> also. We have an inhibitor of this enzyme, which we are keen to
> visualise by X-ray methods.
> We seem to have very strong density in which we can model our
> inhibitor, with good stats and no negative density. However, there is
> only density in one of the monomers, nothing in the other. The SG is
> P212121, and although I can postulate why this may have happened (if
> this is indeed what HAS happened), different solvent channel
> accessibility etc., I would like to know how common this was and, if
> possible, some literature regarding this, if the board would be so
> kind?
>
>
> Regards,
>
> Andrew.
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