Can you send your fragment of the pdb containing the ligand, and the cif
file?
Eleanor
Yahui Yan wrote:
> Hello,
>
> Could you please help me with the sketcher?
>
> I'm trying to use ccp4 sketcher to generate a new ligand and then complex it
> with a protein in coot. I've drawn the ligand, numbered each atom and
> defined each bond type. Then I ran save file, create library description.
> The pdb file was loaded to coot and worked fine. Then I imported cif file.
> However, when I tried to refine the ligand, a message popped out, saying 'No
> restrains'. I double checked the numbering, I think it's Ok. Did I do
> anything wrong? I'm really struggling on this. If you need more information,
> please let me know. Thanks very much.
>
> Best regards,
> Yahui
>
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