Hi Lori,
If you want to get the contribution of a metal ion you have to take the
metal co-ordination into account, so you need to know the fold. A
theoretical pI becomes quite useless, so you'd have to do an IEF gel or
something like that to get your answer.
Flip
Lori Horton wrote:
> Does anyone know of a program that can calculate the theoretical PI of metal-bound proteins? All that I’ve seen are programs that only account for the protein sequence. Any information is helpful. Sorry for the basic non-ccp4 question.
>
>
>
> LH
>
>
>
> Lori B. Horton, Predoctoral Fellow
>
> Baylor College of Medicine
>
> [log in to unmask]
>
|