Thank you for your answer.
Unfortunately, the problem is that analysis does not discriminate, in
this table, between assigned and unassigned sequence positions any
longer. Everything is marked as assigned (blue). I can perfectly ignore
it - because the probability for a correct suggestion seems indeed quite
low until you haven't got a unique stretch - and assign an aa to a
sequence position via the "Spin Systems" panel as I did before. It just
seemed a little bit strange to me that the "Sequence Locations" table
does not work correctly any more.
Regards,
Marcel
Wayne Boucher wrote:
> Hello,
>
> The "Sequence Locations" table is a suggestion of where in the
> sequence the selected spin system might belong, so in your case it
> thinks that spin system {60} might be 187 Asp. That is based on how
> well the shifts within the spin system match the chemical shifts in
> the RefDB database (I don't know the detail, although Tim does). So
> it is not based on the current assignment (the way I understand it).
> (Tim and Rasmus are in Portugal this week giving a course so hard to
> ask them.)
>
> If you don't want to use that prediction then you can just ignore it.
> (I don't know how high the score has to be before one might believe
> it.) So you can just use the "Set Seq Link" functionality in the
> middle table.
>
> Wayne
>
> On Tue, 1 Jun 2010, Marcel Jurk wrote:
>
>> Hi everyone,
>>
>> I am, once again, encountering a strange problem during the course of
>> assignment. Since this morning, analysis shows all sequence positions
>> as already assigned in the "Sequence Locations" menu on the bottom
>> left on the "Protein Sequence Assignment" panel
>> (assign_locations.tiff), although none of these positions is actually
>> assigned. Assignment status is displayed correctly under
>> "Chart-->Assignment Graph" (see assign_graph.tiff).
>> If a new spin system is assigned to one of the positions with
>> misassigned status, analysis considers it indeed as assigned and asks
>> for a merge of both spin systems (non-existing and new). Upon this,
>> sequential links are disrupted regardless of choosing merge or no merge.
>>
>> Everything ran smoothly until today. I installed all the latest
>> updates last Sunday but hadn't had a problem yesterday. Any
>> suggestions as towards the root of this evil are very much appreciated.
>>
>> Many thanks in advance.
>>
>> Regards,
>> Marcel
>>
>> --
>> Marcel Jurk
>>
>> Leibniz Institut fuer Molekulare Pharmakologie (FMP)
>> Solution NMR (AG Schmieder)
>> Robert-Roessle-Str. 10
>> 13125 Berlin
>> Germany
>>
>> eMail: [log in to unmask]
>> Phone: +49-30-94793223
>> Fax: +49-030-94793169
>>
>>
--
Marcel Jurk
Leibniz Institut fuer Molekulare Pharmakologie (FMP)
Solution NMR (AG Schmieder)
Robert-Roessle-Str. 10
13125 Berlin
Germany
eMail: [log in to unmask]
Phone: +49-30-94793223
Fax: +49-030-94793169
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