Usually, this means that the linear fit of a straight line to the
automatically-selected "good" region of your Wilson plot produced a
slope that was ... well. wrong.
Have a look at your Wilson plot (graph log(mean(Intensity)) vs
(0.5/d-spacing)^2) in your favorite graphing program, and then
superimpose on this the lines corresponding to your "alternative"
average B factor choices. I.E. scale*exp(-B*(0.5/d-spacing)^2) where
"scale" is arbitrary. Then you simply ask yourself the question: which
line fits the data better?
-James Holton
MAD Scientist
On 6/30/2010 10:31 AM, Vandu Murugan wrote:
> Dear all,
> If one could find a difference of more than 15 between Wilson B
> factor of the data ( 55) and Mean B factor of the structure, (30) what
> could be the possible reasons? I am seeing it in my structure. Could
> someone tell me why it could be?? Thanks in advance.
>
> Yours faithfully,
> Murugan
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