Dear all,
Does anyone have an idea what this density at a lysine residue could be?
The structure is a protein-DNA complex and I suspect that something
left-over from the DNA synthesis reacted with the amino group even
though the DNA substrate was purified.
christian
p.s. The protein has seen the following substances during purification
and crystallization: Leupeptin, Pepstatin, AEBSF, MES, Tris, HEPES, DTT,
EDTA, imidazole, glycerol, PEG 2K-MME, Mg2+, Na+, K+, Ca2+, Cl-.
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Dr. Christian Biertümpfel
Laboratory of Molecular Biology
NIDDK/National Institutes of Health phone: +1 301 402 4647
9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 0201
Bethesda, MD 20892-0580
USA
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