On 15:52 Tue 25 May , Clayton, Gina Martyn wrote:
> I wonder if anyone can recommend a good review/paper describing
> crystal structures that show high energy residues in active sites. By
> that I mean residues that may be in a strained conformation and rotate
> between conformations, such that they may even switch into unfavoured
> ramachandran regions during their activity, but that the strained high
> energy state is relevant to the enzyme activity?
Gina,
Here are a few papers discussing strained residues in active sites:
Xu, Q., Buckley, D., Guan, C., Guo, H.C. "Structural insights into the
mechanism of intramolecular proteolysis." Cell 98, 651-661 (1999).
Lawson, C.L. "An atomic view of the L-tryptophan binding site of trp
repressor." Nat. Struct. Biol. 3, 986-987 (1996).
Herzberg, O., Moult, J. "Analysis of the steric strain in the
polypeptide backbone of protein molecules." Proteins: Struct. Func.
Bioinf. 11, 223-229 (1991)
Merritt, E.A. et al. "The 1.25 Å resolution refinement of the cholera
toxin B-pentamer: evidence of peptide backbone strain at the
receptor-binding site." J. Mol. Biol. 282, 1043-1059 (1998).
--
Thanks,
Donnie
Donald S. Berkholz, Postdoctoral research fellow
James R. Thompson lab, Physiology & Biomedical Engineering
Grazia Isaya lab, Pediatric & Adolescent Medicine
Medical Sciences 2-66
Mayo Clinic College of Medicine
200 First Street SW
Rochester, MN 55905
612-991-1321
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