Hmm - that is odd.
You may have the wrong SG in the mtz file. Try MOLREP or PHASER with the
option to try all spacegroups consistewnt with the pointgroup.
Eleanor
intekhab alam wrote:
> Hi all
> I am trying to do molecular replacement with low resolution (4Å) using
> Molrep and Phaser.
>
> Overall R-factor is 11.3%, completeness 95.4%, I/sigma 2, and Chi^2 0.95.
>
> Identities between my protein and templates were more than 80%.
>
> I couldn’t get correct solution.
>
> Rotation function, translation score, and contrast were low, and they had no
> significance, though I changed the range of resolution.
>
> Molrep suggested solution coordinates clashed with symmetry molecules.
>
> I tried MR after remove clashed regions, but another clashes happened.
>
> In the case of phaser, there were many clashes, too.
>
> Please, give me any suggestion.
> Should I concern about any options when I run MR programs?
>
> Hope you guys will be interested to answer!!!!!!
> Thanks in advance
>
>
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