Dear All,
does anyone have any clever suggestion/s for handling the solubility problem
of highly hydrophobic compound, during co-crystallization or inhibition
assays? The ligands I am using are almost insoluble in aquous medium
(precipitates out or becomes turbid at ~1 micro molar conc.). In DMSO, the
solubility is upto 50mM. Ligands are not soluble in MeOH or EtOH.
Besides crystallization, this solubility is also a hindrance for
in-vitro or in-vivo assays requiring higher conc. of ligand (keeping in
mind, the final DMSO conc. <5%)!
Some of the ligands have benzoic acid groups. Does it help to convert them
to Na benzoate form?
Thanks for your time,
Tuhin.
Dept. of Physics
Indian Institute of Science.
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