Tuhin,
I have had some success with low molecular weight PEG eg. PEG200.
-bob
On Thu, May 13, 2010 at 1:00 PM, Tuhin Bhowmick
<[log in to unmask]> wrote:
> Dear All,
> does anyone have any clever suggestion/s for handling the solubility problem
> of highly hydrophobic compound, during co-crystallization or inhibition
> assays? The ligands I am using are almost insoluble in aquous medium
> (precipitates out or becomes turbid at ~1 micro molar conc.). In DMSO, the
> solubility is upto 50mM. Ligands are not soluble in MeOH or EtOH.
> Besides crystallization, this solubility is also a hindrance for
> in-vitro or in-vivo assays requiring higher conc. of ligand (keeping in
> mind, the final DMSO conc. <5%)!
> Some of the ligands have benzoic acid groups. Does it help to convert them
> to Na benzoate form?
> Thanks for your time,
> Tuhin.
>
> Dept. of Physics
> Indian Institute of Science.
>
>
>
>
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