Can you soak the crystal in a solution lacking 0.2M lithium sulfate
(since it isn't your precipitant)-perhaps add 0.2M sodium chloride to
maintain ionic strength- then in a high concentration of your ligand
solution?
Nat
On Fri, Apr 23, 2010 at 3:26 PM, Jim Pflugrath <[log in to unmask]> wrote:
> I would test several hypotheses. You have a multitude of salts to choose
> from. Test them all.
> Let me ask you this: What anions can you test? What's already on your
> shelf of chemicals? What did you test already?
>
> Jim
> ________________________________
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> Yi-Liang Liu
> Sent: Friday, April 23, 2010 12:53 PM
> To: [log in to unmask]
> Subject: [ccp4bb] Substitute for Lithium Sulfate
>
> Hi Everyone,
>
> I have a question about the crystallization condition. Currently the
> condition I use contains 0.2M lithium sulfate. However, the ligand there
> seems to compete the site where sulfate binds in the active site. Are there
> any good substitute to replace the Lithium sulfate in my crystallization
> buffer?
> Best,
> Lucas
>
>
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