Dear all;
I am working with protein which are supposed to be bound with Magnesium
and Cobalt metals.I have now its crystals which are diffracting quite
nicely.I want to know the Magnesium and Cobalt binding residues in the
target protein. When I add magnesium or Cobalt salts to protein solution
even 1mM about 50 percent of the protein precipitated out. When I soaked
the crystals in about 10mM of the metals salt it lose diffraction. I
varied the soaking time and the metal salt concentration,and even the
types of metals salts as well but its seems that it does n,t work.
Any idea would be highly appreciated.
Bashir
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Muhammad Bashir Khan
Department for Biomolecular Structural Chemistry
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
Phone: +43(1)427752224
Fax: +43(1)42779522
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