Amit Sharma,
Since the type of information you are trying to get is really basic, you
don't need a cat wisker, use an eyelash or eyebrowse instead (not nylon).
To make a probe you can use common laboratory materials. (see illustration)
Enrico.
On Tue, 13 Apr 2010 16:23:52 +0200, Tim Gruene <[log in to unmask]>
wrote:
> On Tue, Apr 13, 2010 at 09:24:13AM -0400, Nathaniel Clark wrote:
>> [...] If you don't want to buy those tools just try 2 nylon loops, and
>> lots
>> of patience.
> Or use cats whiskers which you can fix into a Pasteur pipette with wax,
> nail
> varnish, plasticine, etc.
>
> I find them to have just the right stiffness to work with crystals, the
> tip is
> very pointy and does not fray.
>
> You just need to know a cat owner and ask them to look out for the
> whiskers the
> next time they vaccuum clean their flat.
>
> Tim
>
>> Nat
>>
>> On Tue, Apr 13, 2010 at 4:49 AM, Enrico Stura <[log in to unmask]> wrote:
>> > Amit Sharma,
>> >
>> > I second Tim's suggestions.
>> >
>> > The first experiment is the following.
>> > Set up several experiments at constantly decreasing precipitant
>> > concentrations.
>> > Streak seed all of them. If in any of those you can get non-clustered
>> > crystals
>> > non matter what size, seeding can solve your problem.
>> > To restore crystal size follow the rest of Tim's list.
>> >
>> > Enrico.
>> >
>> >
>> > On Mon, 12 Apr 2010 23:01:10 +0200, Tim Gruene
>> <[log in to unmask]>
>> > wrote:
>> >
>> >> Have you tried anything, yet? The list of answers can be quite long.
>> >> A couple of keywords:
>> >> - micro-seeding
>> >> - macro-seeding
>> >> - cross-seeding
>> >> - additive screens
>> >> - extend to purification protocol
>> >> - change temperature, pH, etc.
>> >> - have you searched around the current conditions
>> >> ...
>> >>
>> >> if you describe a little what you have done so far we can also get
>> an idea
>> >> of
>> >> your status of experience and adapt the answers accordingly.
>> >>
>> >> Kind regards, Tim
>> >
>> > --
>> > Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
>> > Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
>> > LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
>> >
>> http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
>> > http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
>> > e-mail: [log in to unmask] Fax: 33 (0)1 69 08
>> 90 71
>> >
>
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
|