Hampton's CryoPro has two 2,3-Butanediols: ## 25 and 26.
So, which one is not the right one for cryo-preservation?
Vaheh
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Leiman Petr
Sent: Wednesday, March 24, 2010 12:37 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] Butandiol as Cryoprotectant 2.
>
> thanks so far for sharing your experience with Butanediol. But is it
> important to use a specific enantiomer ? Are you all only using 2R3R-
> Butanediol which is quite expensive ?
Sigma Cat. # 18970
2,3-Butanediol puriss., mixture of racemic and meso forms, ™99.0% (GC) (Fluka)
works well and freezes clear.
And it's cheap!
We've used it recently to collect 1.6 A resolution data from a crystal with 2000+ residues in the asymmetric unit.
Petr
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