Pentapeptide ligand removed from the binding site of the protein crystal and
replaced with different peptide ligand
Insights into Cyclin Groove Recognition: Complex Crystal Structures and
Inhibitor Design through Ligand Exchange, Kontopidis, G., et. al.
2003,Structure 11 (12), pp. 1537-1546
George
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Critton, David
Sent: Wednesday, March 17, 2010 6:02 PM
To: [log in to unmask]
Subject: [ccp4bb] Soaking to Remove Bound Ligands from Crystals
Dear ccp4bb,
I recently came across a thread from 2007 discussing the removal of
ions/ligands from protein crystals
(https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0705&L=CCP4BB&P=R35929).
The thread cited a couple of papers (Ray et al. 1991; Schreuder et al. 1988)
in which crystals were grown in the presence of a given ligand, then soaked
in conditions containing little (if any) ligand in order to remove the
ligand from these crystals.
I would like to know if there are any other reports of this method,
especially if any structures were solved from pre- and post-soaked crystals,
and what kinds of conformational changes (if any) accompany these kinds of
soaks.
Thank you in advance,
David A. Critton
Graduate Student, Page Laboratory
Department of Molecular Biology, Cell Biology & Biochemistry
Brown University
Providence, RI
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