I would not rely on recipes. When I pick a cryo protectant, I always prepare a
set of solutions and take an image from a large loop dipped into solution.
Then I pick the one with the least concentration that does not show ice rings
anymore.
The behaviour can change with a crystal in the loop, but I hope by using a large
loop and taking a big drop I'm on the safe side.
For 2,3-butanediol I'd probably prepare the mother liquor with 10%, 15%, 20%
2,30butanediol. Having read what Eike just replied I might include the
"2.5%"-steps, too.
Tim
On Tue, Mar 23, 2010 at 07:27:28AM +0000, David Briggs wrote:
> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delivered via an Android.
>
> On Mar 22, 2010 11:45 PM, "Steve Tuske" <[log in to unmask]> wrote:
>
> Hi Ulrike,
>
> 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
> mostly seems to decrease mosaicity. I was able to switch from a
> three-step soaking protocol with glycerol and glucose to a quick (30
> second to one minute) soak with 2,3-BD.
>
> It's a winner.
>
> Steve
>
> I
>
> > Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. > >
> Tim > > On Mon, Mar 22...
--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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