Dear all,
I would like to know which is the more suitable method of using 2,3-Butanediol as cryoprotectant? Addition in to reservoir buffer or crystal soaking or both can be tried?
Cheers
Gauri
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I am sure both could be tried, but have found quite few papers mentioning brief soaking with 2,3-butanediol:
Purification, crystallization and X-ray diffraction analysis of the extracellular part of the human Fc receptor for IgA, Fc[alpha]RI (CD89)
http://scripts.iucr.org/cgi-bin/paper?cnor=bw5002
5-10 seconds
Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus
http://scripts.iucr.org/cgi-bin/paper?cnor=gr2202
described as 'briefly'
Crystallization and preliminary X-ray analysis of recombinant human acid [beta]-glucocerebrosidase, a treatment for Gaucher's disease
http://scripts.iucr.org/cgi-bin/paper?cnor=pu0086
described as 'briefly'
Crystallization and preliminary X-ray crystallographic studies of recombinant human betaine-homocysteine S-methyltransferase
http://scripts.iucr.org/cgi-bin/paper?cnor=gr2115
'quickly transferred'
Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus
http://scripts.iucr.org/cgi-bin/paper?cnor=pu0115
5% increments from 5 to 30% for 3 min at each step
Hope that helps,
Sean
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