Miguel Ortiz Lombardia wrote:
> Le 4 févr. 2010 à 04:37, Norman Zhu a écrit :
>
>
>> hello there
>>
>> I am in the process of refining a protein structure complexed to DNA
>> promoter site. I ran into difficulty as i try to move a few bases into
>> patch of electron density that is obviously meant for them. dragging the
>> bases with real space refine zone and/or regularize zone neither break open
>> the phosphate back bone bounds or turn everything into knots.
>> I know there is nothing wrong with the naming convention of my bases
>> since I changed them from DT to Td after similar blog.
>> Any suggestion on this matter would be greatly appreciated.
>>
>> Norm
>>
>>
>
> Hi Norm,
>
> Depending on the resolution of your data you may need to turn down the matrix weight in refinement parameters. I tipically put this down to 30 (compared to the default value of 60) and for really lower resolution (say 3 or worse) I may go as down as 20.
>
>
I frequently use 6.0 or even 2.0 sometimes.
Paul
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