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Subject:
Expression of a protein of 43KDa
From:
Armando Albert de la Cruz <[log in to unmask]>
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Date:
Thu, 18 Feb 2010 18:49:57 +0100
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I am trying to overexpress a His-tagged protein of 29KDa in E.coli (BL21-codon plus) and I end up with a highly expressed product that of 43KDa that binds to the Ni-column. I also have nice crystals. Does anyone have any experience on this. Armando
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