Dear Lalit,
There are two issues here: How to describe the experiment types, and how
to describe the molecule labelling state. On the topic of the first, could
you describe exactly what each experiment does? What is on each axis,
what is covalently bound to what, and what the pulse sequence does (HSQC,
double-half finlter, ...)? I could make a good guess, but it is too easy
to make misunderstandings here.
We shall be coming back about how to specify the labelling state.
Yours,
Rasmus
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Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Thu, 21 Jan 2010, Lalit Deshmukh wrote:
> Hi,
>
> I am working with protein-peptide complex in Analysis. For the structure
> calculations of the complex, I have three sets of spectra collected on 13C,
> 15N labeled protein+ non-labeled peptide sample:
>
> a) 3D 15N-edited and 13C-edited NOESYs
> b) 2D and 3D 13C, 15N filtered-NOESYs
>
> When I define my Analysis project, I say Protein is chain A and Peptide is
> chain B. How to set up the Analysis project to distinguish between inter and
> intramolecular NOEs for these edited and filtered NOESY spectra?
>
> Thanks in advance,
>
> Regards,
> Lalit
>
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