Dear Muhammed,
that's not uncommon. Your protein might undergo domain movements or
contain flexible parts.
if your structure contains any loops / floppy regions (with high
B-values), you can exclude them from the search model.
If it is composed of domains, chop it into pieces and search with one
after the other, starting from the largest one.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Mon, 18 Jan 2010, Muhammed bashir Khan wrote:
> Dear All;
>
> We have solved a crystal structrure of protein at 1.8 A. I have now
> another crystal of the same protein in aother unit cell,for the new
> crystal type resolution is 3.6 A but when I use our structure as a seach
> model It does't give any solution.
>
> Any suggestion would be highly appreciated.
>
>
> --
> Muhammad Bashir Khan
> Department for Biomolecular Structural Chemistry
> Max F. Perutz Laboratories
> University of Vienna
> Campus Vienna Biocenter 5
> A-1030 Vienna
> Austria
>
> Phone: +43(1)427752224
> Fax: +43(1)42779522
>
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